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Not necessarily authorized sequel to "Sweetie Belle's Gift" and "Apple Bloom Lends a Hand, and Other Limbs" (since I haven't heard from their authors). Tags: Female/Female; snuff; dissection; cannibalism; non-consensual.

***
Twilight hummed contentedly to herself as she moved around the laboratory of her castle, setting up the equipment she'd need. After all, this was a very special day. Once everything was in place, she started into the room next door, which she had recently and officially dubbed "The Brain Room". For it was here that the still living brains of Equestrians who had, for whatever reason, allowed their bodies to be taken apart after a special spell was cast, allowing their brains to survive regardless of the condition of their bodies.

The top shelf held two very special brains: those of Sweetie Belle and Apple Bloom, the younger sisters of Twilight's friends Rarity and Applejack, who had both consented to letting their older sisters cut them up and use their parts for other purposes. Sweetie Belle had become the basis for Rarity's new line of leather goods, while Apple Bloom's family had used her parts for a variety of purposes, including turning her bones into farm tools. Next to them sat an empty jar labeled with the name "Scootaloo", reserved for the last living member of the original Cutie Mark Crusaders, whom Twilight expected would be joining her friends any day now. And on the same shelf, but some distance away, sat two empty jars reserved for Diamond Tiara and Silver Spoon, two bullying fillies from Ponyville whose parents had quietly confessed to Twilight that they planned to sell them to Rarity for parts if they didn't shape up soon, and that she was more than welcome to their brains once they were harvested.

That led to another shelf, which had yet to be occupied, but that would change in just a few hours. Soon after Sweetie Belle's brain had come to stay with her, Twilight had approached her fellow princesses with a proposal. Crime was rare in Equestria, and crimes worthy of the death penalty even rarer, to the point where even when it happened, the ones who committed them were merely thrown in prison.

But months ago, a criminal had surfaced who was simply too dangerous to be imprisoned by normal means. Starlight Glimmer, an extremely talented unicorn, had been found running a village occupied entirely by ponies with the same Cutie Mark. Twilight and her friends had discovered that Starlight was in fact using a spell to strip the residents of their original marks and replace them with a new one that suppressed all individual talents and personality traits. Once her actions were exposed, Starlight had evaded capture and fled to parts unknown.

After returning to Ponyville, Twilight had alerted the royal guard, informing them of Starlight and her actions, and they had eventually caught her. Because of her crimes, the two senior princesses had reluctantly agreed to Twilight's proposed plan: harvest Starlight and reduce her to a brain in a jar so she could never hurt anypony ever again like she had before. Furthermore, when Twilight had suggested that she could also harvest other ponies guilty of heinous crimes, using her castle as a sort of prison for them once they were just brains in jars, Luna and Celestia had agreed to that as well. And now, Starlight was due to be delivered to her castle within the day, while at least two more would arrive by the end of the week.

"Miss Twilight?"

Twilight blinked as she was startled out of her introspections, and turned to the younger girl at the door. "Yes, Liza?"

"They're here."

"Ah, good. Thank you, Liza." Twilight watched the younger girl go with a smile. A week or so before, Liza's parents, an ill and aging couple who didn't have long for the world, had come to the castle and offered to sell their daughter to Twilight, knowing she would take good care of a growing child. Twilight had consented, and had made Liza her new assistant. (Spike, who normally held the position, had recently moved to the Crystal Empire to help Cadance and Shining Armor take care of their own newborn daughter, and Owlowiscious‎, useful as he was, had his own limitations that kept him from taking over for Spike full-time.) She had done a wonderful job so far, but today would be her biggest test yet: seeing how she handled herself during the dissection of another living being. If she did well enough, Twilight had promised to begin teaching her more skills, effectively making her the princess's apprentice.

A short while later, everything was ready, as Starlight lay on the table, stark naked and magically bound to prevent movement as Twilight and Liza stood over her. A second restraint had been placed on her horn to prevent any magical discharges, and Twilight had just placed the preservation spell on her brain, before turning to Liza. "Now, we're going to remove her horn first, so here's what we need to do." Pressing the scalpel gently against the skin at the base of the horn, she continued talking. "You have to gently press the blade against the skin, and sort of roll it along around the outer edge, so you don't accidentally do any damage to the horn itself, understand?"

Liza nodded as Twilight continued cutting. "Once you have the incision made all the way around, you cut just a little deeper and then... out it comes!" She extracted the horn, and the carbuncle just underneath it, and placed the items into the jar Liza had ready for her, then set them aside. "Now, we apply the sealing spell so she doesn't bleed out, and flip her over so we can start skinning her." Starlight's eyes widened (in spite of the spell on her), as Twilight continued. "We need to be careful here too, because Rarity wants her skin for her shop, and it has to be perfect." She examined Starlight's body, and pointed to a spit. "We'll start the cut... here!" She sliced gently into the skin, pointing out key spots where they would cut again, so the skin would ultimately come off in one piece, and even let Liza have a go at it in some of them, watching proudly as the younger girl's hand moved steadily, never wavering and allowing her to make the cuts without a single mistake. Between them, they soon had the entire skin off, and placed under a separate preservation spell so Rarity could treat it properly after she got it back to her shop.

With the skin removed, the two now moved on to the next stage, opening up the body and removing the internal organs. Twilight watched as Liza cut through the layers of muscle on Starlight's torso, cutting it into steaks and setting it aside. Once all the meat had been removed from her front, they were able to get to the organs below and gently cut them loose, storing each of them in their own sample jars. "These will go to Fluttershy," Twilight pointed out as they took out the stomach, esophagus and entrails. "The liver, kidneys and pancreas are all suitable for transplant into those who need them, and the heart and lungs too, once we get those out."

Next to come were the ovaries and uterus, and Twilight watched as Liza gently cut them out. "These, I have something special in mind for," she told Liza. "Rarity gets the womb, but the ovaries are staying here."

"What are you going to do with them?" Liza asked.

"That..." Twilight grinned mischievously, "Is a secret!"

Finally, only the heart and lungs remained, but Twilight had something special in mind first. "Now, we're going to take out those eyes." (If it had been possible, they would have widened further in horror.) "This is one of the most critical parts of the operation, and there's a very specific ritual that has to be done during it. First, for this to work properly, her own reflection has to be the last thing she sees." She pointed to the hand mirror nearby. "I'll be holding that up while you take out the eyes. Then, we pack them in myrrh, cassia bark and aloes." She held up a jar of green material. "That's right here. I have a spell I have to say afterward, and then we set them aside for forty days while they sort of... crystalize. Once they're all done, they can be used for something very important. Now, eyes can be damaged very easily, so be very, very careful. Ready?"

Liza nodded, and slowly, carefully worked her way in behind the left eye before severing the nerve holding it into the socket. She set the eye in the jar, then took out the other one. Twilight, setting the mirror aside, quickly packed the eyes in the jar's mossy contents, muttering a spell under her breath, before sealing the jar. "There. Now, for the piéce de résistance, the brain itself."

"Um, what about her heart and lungs?"

Twilight waved a hand flippantly. "They come out afterward. So, what we do, is cut the rest of the muscle tissue off her head."

Liza followed her teacher's directions, gently slicing the rest of the meat off of Starlight's skull and setting it aside, before they began opening up the skull itself. Working carefully, the two managed to remove the brain, stem and all, and place it into the jar Twilight had prepared. Looking at the brain, Twilight smiled, and pointed to the next jar. "Now, the heart and lungs." She pointed to it, and Liza got to work, cutting the last organs from Starlight's torso. Once they were stored, Twilight gestured to the body. "I'm going to take all these jars out, and put them somewhere safe. You can take the rest of the meat off her bones while I'm doing that."

Liza smiled, and went back to work. While Twilight bustled around with the other jars, she stripped the muscle tissue from the bones, and soon had it all neatly set aside. Once Twilight returned, she wrapped up the meat into separate bundles for storage in her kitchen's freezer. They would make for plenty of good eating in the days to come. While she was doing that, Liza finished the last of the cleaning of the bones themselves.

A few hours later, as Twilight checked the Starlight steaks she had put on to grill, she looked at Liza, who was standing nearby. "You did a wonderful job today, Liza."

"Th-thank you, Miss Twilight."

"In fact, you did such a wonderful job..." Twilight smiled. "You passed my test. You're my new apprentice, Liza."

The younger girl looked happy. "Really?"

"Really." Twilight pulled her into a hug. "I can't imagine anypony I'd rather have at my side for this job. And I'm sure you're going to do just as well on the next few ones who come in."

Liza smiled. "So, what are they in for?"

"Well, there's one named Suri Polomare. She's been involved in some... fraudulent business practices, and she's been caught at it too many times, with no signs of remorse. They're sending her from Manehatten in just a few days. The other is a mare from Cloudsdale, and she's the big one."

"Oh?"

Twilight nodded. "Lightning Dust is guilty of reckless flying that caused severe injuries to other ponies, putting them in the hospital. One of them was the daughter of the mayor of Cloudsdale, which is why he pushed for her to be sent to me for harvesting. She'll be here in a week."

"Ouch."

"Very ouch. And now... can you set the table? The others will be here soon, and I need things to be ready when they arrive."

"Okay!" Liza ran off.

Twilight smiled as she watched the younger girl go. Ah, to be that young again... She turned back to the steaks, and flipped them. Taking her in was the best decision I ever made. Maybe I'll get really lucky, and she'll wind up an alicorn like me. Then we can keep doing this for a very, very long time.

THE END

***

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